Graham Delafield, Ph.D.

228 Duncan Street · San Francisco, CA 94131 · (405) 219-4741 · graham.delafield@gmail.com

Below is an up-to-date repository of my scientific contributions, posters and presentations. While all posters and presentations may be viewed and saved from the browser, the journal publications are left as links to the publishers. Should you be interested in reading further and cannot access the material online, please contact me and I will happily provide the entire manuscript.


Publications

JPR Graphical Abstract
Inclusion of Porous Graphitic Carbon Chromatography Yields Greater Protein Identification, Compartment and Process Coverage, and Enables More Reflective Protein-Level Label-Free Quantitation

Journal of Proteome Research

Efforts describing the extent to which utilizing PGC separations recover proteomic information typically lost during RPLC analyses, even with reduced sample complexity.

Read
JASMS Graphical Abstract
Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes

Journal of the American Society for Mass Spectrometry

Investigations of the optimal chromatographic conditions that promote identification efficiency and optimal liquid phase resolution.

Read
TrAC Graphical Abstract
High-End Ion Mobility Mass Spectrometry: A Current Review of Analytical Capacity in Omics Applications and Structural Investigations

Trends in Analytical Chemistry

Review of high-end ion mobility instrumentation and their suitability in metabolomics/proteomics investigations, as well as their capacity for structural elucidation.

Read
ABC PGC TOC Figure
Complementary proteome and glycoproteome access revealed through comparative analysis of reversed phase and porous graphitic carbon chromatography

Analytical & Bioanalytical Chemistry

First reports of porous graphitic carbon for untargeted glycoproteomics compared to traditional reversed phase liquid chromatography.

Read
RSC-ChemBio Review Image
Recent developments and applications of quantitative proteomics strategies for high-throughput biomolecular analyses in cancer research

RSC Chemical Biology

Review of recent applications and associated advancements in quantitative proteomics applications in cancer research.

Read
MCP Review Image
Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation

Mollecular & Cellular Proteomics

Review of experimental considerations and advancements for quantitative glycomics and glycopeptidomics over the years 2010-2020.

Read
Anal. Chem. Aminophospholipids
sn-position Resolved Quantification of Aminophospholipids by Isotopic N, N-Dimethyl Leucine Labeling and High-resolution Ion Mobility Mass Spectrometry

Analytical Chemistry

Extension of the previously-developed IM-MS method to distinguish structural peptide isomers into aminophospholipid analysis.

Read
Nat. Comm. Image
Spatially and Temporally Probing Distinctive Glycerophospholipid Alteration In Alzheimer's Disease Mouse Brain Via High-Resolution Ion Mobility-Enabled sn-Position-Resolved Lipidomics

Nature Communications

Ion mobility-based methodology for resolving sn-positional isomers and application to Alzheimer's Disease Mouse model.

Read
Chem Neuroscience Fragile
On-tissue Spatial Proteomics Integrating MALDI-MS Imaging with Shotgun Proteomics Reveals Soy Consumption-induced Biomarkers in a Fragile X Syndrome Mouse Model

ACS Chemical Neuroscience

Evaluation of diet-indeuced proteomic alterations in situ in mouse brains.

Read
An Chem CHRISTMAS
Chiral Pair Isobaric Labeling Strategy for Multiplexed Absolute Quantitation (CHRISTMAS) Enabled Quantitatively Probing of Enantiomeric Amino Acids Alteration in Alzheimer's Disease Progression

Analytical Chemistry

Novel method for D-amino acid quantification using IM-MS.

Read
Chem Sci AB42
Site-specific Chirality-conferred Structural Compaction Differentially Mediates the Cytotoxicity of AB42

Chemical Science

Primary research conferring the significance of D-amino acid participation in AB42 oligomerization and their contribution towards cytotoxicity.

Read
Single Cell IMaging
Mass spectrometry imaging: the rise of spatially resolved single-cell omics

Nature Methods

Commentary on the rapidly growing interest of single-cell biomolecular analyses and discussion over the the analytical merit and biological significance of single-cell mass spectrometry imaging.

Read
Citrullination TOC Image
Enabling Global Analysis of Protein Citrullination via Biotin Thiol Tag-Assisted Mass Spectrometry

Analytical Chemistry

Validation of a novel biotin tag capable of selectively enriching citrullinated peptides from complex samples and the use of mass spectrometry for confident identification.

Read
Nat Methods Figure 1
Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome

Nature Methods

A novel diagnostic ion-based strategy, to confidently identify new lactylation events, unveiling a wide lactylproteome landscape that had previously been confined to lactylated histones.

Read
MCP Review Image
Proteome-wide and Matrisome-specific Alterations during Human Pancreas Development and Maturation

Nature Communications

This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.

Read
Card image cap
Peroxymonosulfate Oxidizes Amino Acids in Water without Activation

ACS Environmental Science

Peroxymonosulfate is capable of oxidizing 19 amino acids without explicit activation and that solvent-exposed methionine and tryptophan residues are likely initial targets of oxidation in peptides and proteins.

Read
Card image cap
Improved Structural Elucidation of Peptide Isomers and Their Receptors Using Advanced Ion Mobility-Mass Spectrometry

Trends in Analytical Chemistry

We explore the usefulness of ion mobility techniques in probing structurally-critical biomolecules, examining the ability of current methods to elucidate neuropeptide stereoisomers and receptors.

Read
Card image cap
Finding Biomass Degrading Enzymes Through an Activity-Correlated Quantitative Proteomics Platform (ACPP)

Journal of the American Society for Mass Spectrometry

The results demonstrated that the ACPP facilitated the discovery of bioactive enzymes from complex protein samples in a high-throughput, multiplexing, and untargeted fashion.

Read

D.G. Delafield, H.N. Miles, T.N. Liu, W.A. Ricke, L. Li. “Inclusion of Porous Graphitic Carbon Chromatography Yields Greater Protein Identification, Compartment and Process Coverage, and Enables More Reflective Protein-Level Label-Free Quantitation.” Published online October 10, 2023. 10.1021/acs.jproteome.3c00373

D.G. Delafield, H.N. Miles, W.A. Ricke, L. Li (2022). “Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes.” Journal for the American Society for Mass Spectrometric. Published online November 30, 2022. 10.1021/jasms.2c00249

D.G. Delafield, C. Kaminsky, G. Liu, L. Li. (2022) “High-End Ion Mobility Mass Spectrometry: A Current Review of Analytical Capacity in Omics Applications and Structural Investigations.” Trends in Analytical Chemistry. Published online August 24, 2022. 10.1016/j.trac.2022.116761

D.G. Delafield, H.N. Miles, Y. Liu, W.A. Ricke, L. Li, (2022). "Complementary Proteome and Glycoproteome Access Revealed Through Comparative Analysis of Reversed Phase and Porous Graphitic Carbon Chromatography." Anal. Bioanal. Chem. Published online February 9, 2022. 10.1007/s00216-022-03934-7

D.G. Delafield†, H.N. Miles†, L. Li, (2021). "Recent Developments and Applications of Quantitative Proteomics Strategies for High-Throughput Cancer Biomolecular Analyses in Cancer Research." RSC Chemical Biology. Published online May 15, 2021. 10.1039/D1CB00039J (†Co-first authors)

D.G. Delafield, L. Li, (2020). "Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation." Molecular & Cellular Proteomics. Published online June 23, 2020. 10.1074/mcp.R120.002095

D.G. Delafield, H.N. Miles, W.A. Ricke, L. Li. "Proteomic Fingerprinting of Prostate Cancer Progression Through Library-Free DIA-MS Reveals Systematic and Conserved Pathway Dysregulation." (In Submission) Poster Available

D.G. Delafield†, X. Zhong†, L. Li. "Sample Agnostic Spectral Libraries Enable Quantitation of >9,300 Cerebrospinal Fluid Proteins Across Neurodegenerative Disease Patient Cohorts." (In Submission) († Co-First Authors) Poster Available

D.G. Delafield, N. Treuttner, H.N. Miles, W.A. Ricke, L. Li. “Capsule Network Retention Time Correction Facilitates Prostatic Tumorigenesis Discovery Through Porous Graphitic Carbon DIA-MS.” (In Preparation)

D.G. Delafield†, X. Zhong†, L. Li. "Enhanced Proteomic Coverage Enabled through BoxCar DIA and Sample Agnostic Spectral Libraries." (In Preparation) († Co-First Authors)

Y. Liu, D.G. Delafield, L. Li "Comprehensive Mass Spectrometric Characterization of Neuropeptidome in Nervous System of the Atlantic Blue Crab, Callinectes sapidus." Analytical Chemistry. (To Be Submitted).

X. Sun, D.G. Delafield, F. Hu, J. Cheng, D.D.Y. Chen, H. Zhang, X. Huang, L. Li, Z. Li, Y. Chen, C.C.L. Wong, "Enhanced Native Glycoproteome Characterization for Immune Disorders Using Ion Mobility-Assisted Mass Spectrometry." In Submission.

S. Xu, Z. Zhu, T.J. Gu, Z. Wang, D.G. Delafield, M.J. Rigby, G. Lu, M. Ma, P. Liu, L. Puglielli, L. Li, "sn-position Resolved Quantification of Aminophospholipids by Isotopic N, N-Dimethyl Leucine Labeling and High-resolution Ion Mobility Mass Spectrometry." Published online December 4, 2024. 10.1021/acs.analchem.4c05107

S. Xu, Z. Zhu, D.G. Delafield, M.J. Rigby, G. Lu, L. Puglielli, L. Li, "Spatially and Temporally Probing Distinctive Glycerophospholipid Alteration In Alzheimer's Disease Mouse Brain Via High-Resolution Ion Mobility-Enabled sn-Position-Resolved Lipidomics." Published online July 24, 2024.

M. Ma, Q. Yu, D.G. Delafield, Y. Cui, Z. Li, W. Wu, X. Shi, A. Gutierrez, P.R. Westmark, M. Xu, C.J. Westmark, L. Li (2023) “On-tissue Spatial Proteomics Integrating MALDI-MS Imaging with Shotgun Proteomics Reveals Soy Consumption-induced Biomarkers in a Fragile X Syndrome Mouse Model.” Publishbed online December 18, 2023. 10.1021/acschemneuro.3c00497

Z. Zhu, S. Xu, Z. Wang, D.G. Delafield, M.J. Rigby, G. Lu, T. Gu, P. Liu, M. Ma, L. Puglielli, L. Li, (2023) "Chiral Pair Isobaric Labeling Strategy for Multiplexed Absolute Quantitation (CHRISTMAS) Enabled Quantitatively Probing of Enantiomeric Amino Acids Alteration in Alzheimer's Disease Progression," Analytical Chemistry. Published online November 30, 2023. 10.1021/acs.analchem.3c03847

G. Li, C.K. Jeon, M. Ma, Z. Zheng, Y. Jia, Z. Zheng, D.G. Delafield, G. Lu, E. Romanova, J. Sweedler, B. Ruotolo, L. Li, (2023) "Site-specific Chirality-conferred Structural Compaction Differentially Rescues the Cytotoxicity of Aβ42." Chemical Science. Published online May 8, 2023. 10.1039/D3SC00678F

H. Zhang, D.G. Delafield, L. Li (2023) "Mass spectrometry imaging: the rise of spatially resolved single-cell omics," Nature Methods 20, 327-330. 10.1038/s41592-023-01774-6

Y Shi, Z. Li, B. Wang, Z. Ye, H. Ye, D.G. Delafield, X. Shi, Z. Chen, F. Ma, L. Li (2022) "Enabling Global Analysis of Protein Citrullination and Homocitrullination via Biotin Thiol Tag-Assisted Mass Spectrometry." Analytical Chemistry. Published online December 13, 2022. 10.1021/acs.analchem.2c03844.

N. Wang, N. Wang, S. Yu, H. Zhang, S. Tang, D. Wang, W. Lu, H. Li, D.G. Delafield, Y. Kong, X. Wang, C. Shao, L. Lv, G. Wang, R. Tan, N. Wang, H. Hao, H. Ye (2022) “Cyclic Immonium Ion of Lactyllysine Reveals Widespread Lactylation in the Human Proteome.” Nature Methods. Published online June 28, 2022. 10.1038/s41592-022-01523-1

Z. Li, D. M. Tremmel, F. Ma, Q. Yu, M. Ma, D.G. Delafield, Y. Shi, B. Wang, S. A. Mitchell, A. K. Feeney, V. S. Jain, S. D. Sackett, J. S. Odorico, L. Li (2020) "Proteome-wide and Matrisome-specific Alterations during Human Pancreas Development and Maturation" Nature Communications. Published online February 15, 2021. 10.1038/s41467-021-21261-w

Ruiz, M., Y. Yang, C. A. Lochbaum, D.G. Delafield, J. J. Pignatello, L. Li and J. A. Pedersen (2019). "Peroxymonosulfate Oxidizes Amino Acids in Water without Activation." Environmental Science and Technology. 53, 10845-10854. 10.1021/acs.est.9b01322

Li, G., D.G. Delafield and L. Li (2019). "Improved Structural Elucidation of Peptide Isomers and Their Receptors Using Advanced Ion Mobility-Mass Spectrometry." Trends in Analytical Chemistry. Published online, June 4, 2019, in press. 10.1016/j.trac.2019.05.048

Ma, H., D.G. Delafield, Z. Wang, J. You and S. Wu (2017). "Finding Biomass Degrading Enzymes Through an Activity-Correlated Quantitative Proteomics Platform (ACPP)." Journal of American for Mass Spectrometry. 28, 655-663. 10.1021/jasms.8b05509


Posters

Card image cap
Revealing Systematic Proteomic Remodeling through Library-Free DIA-MS Analysis of a Novel Progressive Prostate Cancer Cell Model

US HUPO 2023

View Poster
Card image cap
Sample Agnostic Spectral Libraries Enhance Proteomic Coverage in Sample-Limited Data Independent Analyses

Chemistry Research Presentation 2022

View Poster
Card image cap
Evaluation of Porous Graphitic Carbon at Elevated Temperatures for Glycopeptide Analyses: Impacts on Signal Suppression, Reduced Identification and Quantitative Inaccuracies

ASMS 2022

View Poster
Card image cap
High Resolution Demultiplexing Ion Mobility: A New Paradigm for Intact Glycopeptide Structural Assignment

ASMS 2021

View Poster
Card image cap
Enhancing Glycopeptide Detection, Identification, and Structural Characterization through PGC-Incorporated LC-IMS

ASMS 2020

View Poster
Card image cap
Pursuit of Bottom-Up, Middle-Down, and Top-Down Glycoconjugate Analysis Through CE-ESI-MS

ASMS 2019

View Poster
Card image cap
Characterization Analysis of Through Arrical Time Correlation Using Concurrent RPLC Fraction Fraction Monitoring and FAIMS Filtering

ASMS 2018

View Poster
MCP Review Image
Three-Dimensionsal Platform for N-Linked Glycopeptide Separation and Analysis

ASMS 2017

View Poster

Oral Presentations

Thesis Defense Title Slide
Standing in the Way of Proteome Profiling Depth: How the Selection of Chromatography and Mass Spectrometry Data Acquisition Limit Our View of the Proteome

PhD Thesis Defense 2023

WPS 2022
Sample Agnostic Spectral Libraries: An Open Framework For Enhanced Data Independent Analysis Profiling Depth

Wisconsin Proteomics Symposium 2022

View
ACS Fall Meeting 2021
Uncovering Glycoprotein Compositional and Structural Heterogeneity Through Capillary Electrophoresis-Ion Mobility Mass Spectrometry

ACS 2021

View
Pittcon 2021 Oral
Enhancing Glycopeptide Detection, Identification, and Characterization through PGC-Incorporated LC-MS

Pittcon 2021

View
US HUPO 2018 Oral
N-Glycopeptide Feature Identification by Revealing Trends Between Analyte Composition and Compensation Field Through FAIMS-Coupled MS Platform

U.S. HUPO 2018

View